Hotspot

atpB

LSC · 54106-55593 · 1488 bp

Back to Ophiopogon

Pi 0.00323

MarkerSeek score 61.20

Primer available yes

Label atpB

Feature Metadata

label_name: atpB
feature_type: gene
region: LSC
length_bp: 1488
variable_sites: 12
pi: 0.00323
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB_p1	CGTGGTTTGGTAATACGCCA	CGATCAACTTGCTATCGGACA	50.0	47.6	58.6	58.2	1683-1684 bp	1.0	66.6
atpB_p2	GGGGCGTGGTTTGGTAATAC	CGATCAACTTGCTATCGGACA	55.0	47.6	58.9	58.2	1687-1688 bp	1.0	63.8
atpB_p3	CGTGGTTTGGTAATACGCCA	CGATCAACTTGCTATCGGAC	50.0	50.0	58.6	56.4	1683-1684 bp	1.0	63.6
atpB_p4	AGGGGCGTGGTTTGGTAATA	CGATCAACTTGCTATCGGACA	50.0	47.6	59.0	58.2	1688-1689 bp	1.0	63.0
atpB_p5	TAGGGGCGTGGTTTGGTAAT	CGATCAACTTGCTATCGGACA	50.0	47.6	59.0	58.2	1689-1690 bp	1.0	63.0