Hotspot

ccsA-ndhD

SSC · 117309-117531 · 223 bp

Back to Ophiopogon

Pi 0.0318

MarkerSeek score 62.00

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 223
variable_sites: 16
pi: 0.0318
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	TGGGACCCCAAAGAAACTTG	GGATTCTGGACCACGAGAAC	50.0	55.0	57.9	58.0	569 bp	1.0	82.2
ccsA-ndhD_p2	TGGGACCCCAAAGAAACTTG	TTCTCGATGGCTTCTCTTGC	50.0	50.0	57.9	58.0	793 bp	1.0	81.7
ccsA-ndhD_p3	GCCCCAAGTGCCATTTTTAC	GGATTCTGGACCACGAGAAC	50.0	55.0	57.9	58.0	1028 bp	1.0	81.3
ccsA-ndhD_p4	TTTTACCCAAGGTTTCGCCA	GGATTCTGGACCACGAGAAC	45.0	55.0	57.9	58.0	1014 bp	0.8	73.4
ccsA-ndhD_p5	TTTTACCCAAGGTTTCGCCA	TTCTCGATGGCTTCTCTTGC	45.0	50.0	57.9	58.0	1238 bp	0.8	73.2