Hotspot

ndhC-trnV-UAC

LSC · 51031-52670 · 1640 bp

Back to Ophiopogon

Pi 0.005

MarkerSeek score 58.70

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1640
variable_sites: 20
pi: 0.005
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	GGTCCTTCACTAATCGGAGC	AGAAGGTCTACGGTTCGAGT	55.0	50.0	57.8	57.8	1786-1795 bp	1.0	77.5
ndhC-trnV-UAC_p2	GGTCCTTCACTAATCGGAGC	AGCCCAATGCAACAAAATGC	55.0	45.0	57.8	58.5	1868-1877 bp	1.0	75.3
ndhC-trnV-UAC_p3	GGTCCTTCACTAATCGGAGC	GAAGGTCTACGGTTCGAGTC	55.0	55.0	57.8	57.5	1785-1794 bp	1.0	74.8
ndhC-trnV-UAC_p4	GGTCCTTCACTAATCGGAGC	GTTCGAGTCCGTATAGCCCT	55.0	55.0	57.8	58.7	1774-1783 bp	1.0	73.7
ndhC-trnV-UAC_p5	GGTCCTTCACTAATCGGAGC	GAAAGAGCCCAATGCAACAA	55.0	45.0	57.8	57.2	1873-1882 bp	1.0	72.5