Hotspot

trnE-UUC-trnT-GGU

LSC · 31793-32285 · 493 bp

Back to Ophiopogon

Pi 0.0238

MarkerSeek score 61.60

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 493
variable_sites: 21
pi: 0.0238
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	GCATCGACCCAGGAAGAATT	GAACCGATGACTTACGCCTT	50.0	50.0	58.0	58.0	521-692 bp	1.0	86.4
trnE-UUC-trnT-GGU_p2	GCATCGACCCAGGAAGAATT	CCATGGCGTTACTCTACCAC	50.0	55.0	58.0	58.1	500-671 bp	1.0	86.0
trnE-UUC-trnT-GGU_p3	GCATCGACCCAGGAAGAATT	AAGCCCTTTCTTTTCTCGCA	50.0	45.0	58.0	58.0	946-1113 bp	1.0	85.6
trnE-UUC-trnT-GGU_p4	GGATCCTAGTTCGGGACTGA	GAACCGATGACTTACGCCTT	55.0	50.0	57.9	58.0	989-1161 bp	1.0	85.3
trnE-UUC-trnT-GGU_p5	GGATCCTAGTTCGGGACTGA	AAGCCCTTTCTTTTCTCGCA	55.0	45.0	57.9	58.0	1414-1582 bp	1.0	85.0