Hotspot

rpoC1

Genome · 34840-37690 · 2851 bp

Back to Ophioglossum

Pi 0

MarkerSeek score 22.40

Primer available yes

Label rpoC1

Feature Metadata

label_name: rpoC1
feature_type: gene
region: Genome
length_bp: 2851
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 22.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC1_p1	GCCATCGTGAAAACCTCGAA	GAAAAGCGACGGATGTTTGA	50.0	45.0	58.8	57.0	2998 bp	1.0	65.6
rpoC1_p2	GCCATCGTGAAAACCTCGAA	AGAAAAGCGACGGATGTTTG	50.0	45.0	58.8	57.0	2999 bp	1.0	65.5
rpoC1_p3	TCTGCCATCGTGAAAACCTC	AAAAGCGACGGATGTTTGAT	50.0	40.0	58.2	56.3	3000 bp	1.0	65.2
rpoC1_p4	ACCTCGAAAATTCTGTGAGCA	GAAAAGCGACGGATGTTTGA	42.9	45.0	57.9	57.0	2986 bp	1.0	63.3
rpoC1_p5	ACCTCGAAAATTCTGTGAGCA	AGAAAAGCGACGGATGTTTG	42.9	45.0	57.9	57.0	2987 bp	1.0	63.3