Hotspot

rpl16

Genome · 94198-95389 · 1192 bp

Back to Ophioglossum

Pi 0.00168

MarkerSeek score 47.90

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: Genome
length_bp: 1192
variable_sites: 3
pi: 0.00168
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	GAACGTCGTGTGAGAACTGA	GGTTGTAGTAACACCATAGTCCC	50.0	47.8	57.9	58.2	1329-1335 bp	1.0	53.7
rpl16_p2	AACGTCGTGTGAGAACTGAG	GGTTGTAGTAACACCATAGTCCC	50.0	47.8	57.9	58.2	1328-1334 bp	1.0	53.7
rpl16_p3	GAACGTCGTGTGAGAACTGA	GTTGTAGTAACACCATAGTCCCA	50.0	43.5	57.9	57.6	1328-1334 bp	1.0	52.3
rpl16_p4	GAACGTCGTGTGAGAACTGA	TGGTTGTAGTAACACCATAGTCC	50.0	43.5	57.9	57.6	1330-1336 bp	1.0	52.3
rpl16_p5	AACGTCGTGTGAGAACTGAG	GTTGTAGTAACACCATAGTCCCA	50.0	43.5	57.9	57.6	1327-1333 bp	1.0	52.3