Hotspot

ndhD

Genome · 113553-115049 · 1497 bp

Back to Ophioglossum

Pi 0.000445

MarkerSeek score 46.50

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: Genome
length_bp: 1497
variable_sites: 1
pi: 0.000445
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	CCAAATAATTCTCTATACATAAGTGCA	TCGGATCGATTGAAAAGACCC	29.6	47.6	55.9	58.1	1688-1690 bp	1.0	40.1
ndhD_p2	ACCAAATAATTCTCTATACATAAGTGC	TCGGATCGATTGAAAAGACCC	29.6	47.6	55.9	58.1	1689-1691 bp	1.0	40.1
ndhD_p3	CCAAATAATTCTCTATACATAAGTGCA	CGGATCGATTGAAAAGACCCA	29.6	47.6	55.9	58.4	1687-1689 bp	1.0	40.1
ndhD_p4	ACCAAATAATTCTCTATACATAAGTGC	CGGATCGATTGAAAAGACCCA	29.6	47.6	55.9	58.4	1688-1690 bp	1.0	40.1
ndhD_p5	TCTCTATACATAAGTGCAAAATAACT	TCGGATCGATTGAAAAGACCC	26.9	47.6	54.6	58.1	1679-1681 bp	1.0	40.1