Hotspot

atpF

Genome · 26549-27839 · 1291 bp

Back to Ophioglossum

Pi 0.00207

MarkerSeek score 47.60

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: Genome
length_bp: 1291
variable_sites: 4
pi: 0.00207
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	AACTACCGATCTCGTCAGGT	TTTGAGCGGGATTCCTGAAG	50.0	50.0	57.9	57.9	1440 bp	1.0	78.3
atpF_p2	ACTCCTCGATTTGTTTGCGA	TTTGAGCGGGATTCCTGAAG	45.0	50.0	57.8	57.9	1464 bp	1.0	78.0
atpF_p3	ACTACCGATCTCGTCAGGTC	TTTGAGCGGGATTCCTGAAG	55.0	50.0	58.3	57.9	1439 bp	1.0	76.6
atpF_p4	AACTACCGATCTCGTCAGGT	GAGCGGGATTCCTGAAGAAA	50.0	50.0	57.9	57.6	1437 bp	1.0	76.0
atpF_p5	ACTCCTCGATTTGTTTGCGA	GAGCGGGATTCCTGAAGAAA	45.0	50.0	57.8	57.6	1461 bp	1.0	75.7