Hotspot

atpA

Genome · 24979-26505 · 1527 bp

Back to Ophioglossum

Pi 0.000437

MarkerSeek score 46.50

Primer available yes

Label atpA

Feature Metadata

label_name: atpA
feature_type: gene
region: Genome
length_bp: 1527
variable_sites: 1
pi: 0.000437
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA_p1	CCACAGATTCCAGGAACCAC	ACCATGAAAAGCACGACTGA	55.0	45.0	58.2	57.7	1644 bp	1.0	76.6
atpA_p2	CCACAGATTCCAGGAACCAC	GGAACCATGAAAAGCACGAC	55.0	50.0	58.2	57.7	1647 bp	1.0	76.0
atpA_p3	CCACAGATTCCAGGAACCAC	TATCACATTGGCTTGCTCGG	55.0	50.0	58.2	58.3	1665 bp	1.0	76.0
atpA_p4	CCACAGATTCCAGGAACCAC	CTCGGAACCATGAAAAGCAC	55.0	50.0	58.2	57.4	1650 bp	1.0	73.7
atpA_p5	CACAGATTCCAGGAACCACA	ACCATGAAAAGCACGACTGA	50.0	45.0	57.4	57.7	1643 bp	1.0	73.5