Hotspot

psaC-ndhE

Genome · 115410-115474 · 65 bp

Back to Ophioglossum

Pi 0.0103

MarkerSeek score 46.50

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: Genome
length_bp: 65
variable_sites: 1
pi: 0.0103
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	GACAGGCTCTTACGCATTGA	TAGCTACACATCGCAATCGG	50.0	50.0	58.0	57.9	191 bp	1.0	79.3
psaC-ndhE_p2	ACAGGCTCTTACGCATTGAG	TAGCTACACATCGCAATCGG	50.0	50.0	58.0	57.9	190 bp	1.0	79.2
psaC-ndhE_p3	AGGCTCTTACGCATTGAGTG	TAGCTACACATCGCAATCGG	50.0	50.0	58.0	57.9	188 bp	1.0	79.2
psaC-ndhE_p4	CAGGCTCTTACGCATTGAGT	TAGCTACACATCGCAATCGG	50.0	50.0	58.0	57.9	189 bp	1.0	79.2
psaC-ndhE_p5	GACAGGCTCTTACGCATTGA	CAGAAGCAGCCATCGGATTA	50.0	50.0	58.0	57.8	221 bp	1.0	78.3