Hotspot

rps8-rpl14

Genome · 93311-93655 · 345 bp

Back to Ophioglossum

Pi 0.00386

MarkerSeek score 57.90

Primer available yes

Label rps8-rpl14

Feature Metadata

label_name: rps8-rpl14
feature_type: igs
region: Genome
length_bp: 345
variable_sites: 2
pi: 0.00386
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8-rpl14_p1	GAACCTTCGCATTCTTTCGC	ACCGAGAAGGAAATCCGAGA	50.0	50.0	58.1	58.2	519-525 bp	1.0	79.1
rps8-rpl14_p2	CGAACCTTCGCATTCTTTCG	ACCGAGAAGGAAATCCGAGA	50.0	50.0	57.9	58.2	520-526 bp	1.0	78.9
rps8-rpl14_p3	CCTTCGCATTCTTTCGCATG	ACCGAGAAGGAAATCCGAGA	50.0	50.0	58.2	58.2	516-522 bp	1.0	78.6
rps8-rpl14_p4	GAACCTTCGCATTCTTTCGC	GTCACCAACCGAGAAGGAAA	50.0	50.0	58.1	57.8	526-532 bp	1.0	78.3
rps8-rpl14_p5	CGAACCTTCGCATTCTTTCG	GTCACCAACCGAGAAGGAAA	50.0	50.0	57.9	57.8	527-533 bp	1.0	78.0