Hotspot

petA-psbJ

Genome · 77054-77694 · 641 bp

Back to Ophioglossum

Pi 0.00104

MarkerSeek score 46.90

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: Genome
length_bp: 641
variable_sites: 1
pi: 0.00104
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TAGCATCGGTTATTACGGCG	ACGGAGCATATTCGGGATTG	50.0	50.0	57.9	57.8	762 bp	1.0	78.3
petA-psbJ_p2	TAGCATCGGTTATTACGGCG	GGAGCATATTCGGGATTGGG	50.0	55.0	57.9	58.2	760 bp	1.0	78.3
petA-psbJ_p3	TAGCATCGGTTATTACGGCG	TCTACGGAGCATATTCGGGA	50.0	50.0	57.9	57.7	765 bp	1.0	77.4
petA-psbJ_p4	TAGCATCGGTTATTACGGCG	TCGGGATTGGGATCATCTCT	50.0	50.0	57.9	57.6	751 bp	1.0	76.4
petA-psbJ_p5	TTGCGTGTTCAAGGTCTTCT	ACGGAGCATATTCGGGATTG	45.0	50.0	57.7	57.8	790 bp	1.0	76.1