Hotspot

psbD-trnT-GGU

Genome · 46944-47680 · 737 bp

Back to Ophioglossum

Pi 0.00181

MarkerSeek score 47.40

Primer available yes

Label psbD-trnT-GGU

Feature Metadata

label_name: psbD-trnT-GGU
feature_type: igs
region: Genome
length_bp: 737
variable_sites: 2
pi: 0.00181
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbD-trnT-GGU_p1	GCCAGACCAACCCACAAATA	GTGGTAGAGTAACGCCATGG	50.0	55.0	58.1	58.1	900 bp	1.0	79.0
psbD-trnT-GGU_p2	GCCAGACCAACCCACAAATA	AATTATTTCACCGGGAGCCC	50.0	50.0	58.1	57.9	930 bp	1.0	79.0
psbD-trnT-GGU_p3	CGAAACGGTCTCTCCTTAGC	GTGGTAGAGTAACGCCATGG	55.0	55.0	58.1	58.1	880 bp	1.0	79.0
psbD-trnT-GGU_p4	CGAAACGGTCTCTCCTTAGC	AATTATTTCACCGGGAGCCC	55.0	50.0	58.1	57.9	910 bp	1.0	78.9
psbD-trnT-GGU_p5	GCCAGACCAACCCACAAATA	TCAGTGGTAGAGTAACGCCA	50.0	50.0	58.1	58.1	903 bp	1.0	78.8