Hotspot

petN-psbM

Genome · 41586-42213 · 628 bp

Back to Ophioglossum

Pi 0.00106

MarkerSeek score 46.90

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: Genome
length_bp: 628
variable_sites: 1
pi: 0.00106
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	GGCTGCTCTGATGGTAGTCT	TCCCCTACGTACAAACAGCT	55.0	50.0	58.9	58.4	730-732 bp	1.0	70.1
petN-psbM_p2	GGCTGCTCTGATGGTAGTCT	ACCTACCGCTTCTTCACTCA	55.0	50.0	58.9	58.4	750-752 bp	1.0	70.1
petN-psbM_p3	GCTGCTCTGATGGTAGTCTT	TCCCCTACGTACAAACAGCT	50.0	50.0	57.0	58.4	729-731 bp	1.0	69.4
petN-psbM_p4	GCTGCTCTGATGGTAGTCTT	ACCTACCGCTTCTTCACTCA	50.0	50.0	57.0	58.4	749-751 bp	1.0	69.3
petN-psbM_p5	GGCTGCTCTGATGGTAGTCT	TCTTCACTCATCCCCTACGT	55.0	50.0	58.9	57.5	740-742 bp	1.0	68.8