Hotspot

rpoC1-rpoB

Genome · 37691-37716 · 26 bp

Back to Ophioglossum

Pi 0.0256

MarkerSeek score 56.30

Primer available yes

Label rpoC1-rpoB

Feature Metadata

label_name: rpoC1-rpoB
feature_type: igs
region: Genome
length_bp: 26
variable_sites: 1
pi: 0.0256
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC1-rpoB_p1	GTTCGGGAGATGCTAACTGG	CCGGGAATTACGGTCGTTAG	55.0	55.0	58.1	58.2	166 bp	1.0	78.8
rpoC1-rpoB_p2	GTTCGGGAGATGCTAACTGG	GGTCGTTAGCTCCGAATCTG	55.0	55.0	58.1	58.2	155 bp	1.0	78.8
rpoC1-rpoB_p3	TCGGGAGATGCTAACTGGAT	CCGGGAATTACGGTCGTTAG	50.0	55.0	57.9	58.2	164 bp	1.0	78.5
rpoC1-rpoB_p4	TCGGGAGATGCTAACTGGAT	GGTCGTTAGCTCCGAATCTG	50.0	55.0	57.9	58.2	153 bp	1.0	78.5
rpoC1-rpoB_p5	TTCGGGAGATGCTAACTGGA	CCGGGAATTACGGTCGTTAG	50.0	55.0	58.1	58.2	165 bp	1.0	78.1