Hotspot

trnS-GCU-trnC-GCA

LSC · 8551-9316 · 766 bp

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Pi 0

MarkerSeek score 40.00

Primer available yes

Label trnS-GCU-trnC-GCA

Feature Metadata

label_name: trnS-GCU-trnC-GCA
feature_type: igs
region: LSC
length_bp: 766
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnC-GCA_p1	ACGGAAAGAGAGGGATTCGA	GTTTGTTAATGAGGCGGCAC	50.0	50.0	58.2	58.0	937 bp	1.0	78.6
trnS-GCU-trnC-GCA_p2	ACGGAAAGAGAGGGATTCGA	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.2	58.1	917 bp	1.0	78.1
trnS-GCU-trnC-GCA_p3	ACGGAAAGAGAGGGATTCGA	AAAAGGATTTGCAGTCCCCC	50.0	50.0	58.2	58.4	899 bp	1.0	75.9
trnS-GCU-trnC-GCA_p4	CTTTCGTCCACTCAGCCATC	GTTTGTTAATGAGGCGGCAC	55.0	50.0	58.6	58.0	873 bp	1.0	74.7
trnS-GCU-trnC-GCA_p5	AACGGAAAGAGAGGGATTCG	GTTTGTTAATGAGGCGGCAC	50.0	50.0	57.3	58.0	938 bp	1.0	74.4