Hotspot

trnK-UUU-rps16

LSC · 4350-5145 · 796 bp

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Pi 0
MarkerSeek score 40.00
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
796
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CCGATGGTATGGACGAATCC TCCTTGAAAAAGGGGCTCAG 55.0 50.0 57.9 57.7 992-993 bp 1.0 77.0
trnK-UUU-rps16_p2 CGATGGTATGGACGAATCCC TCCTTGAAAAAGGGGCTCAG 55.0 50.0 57.9 57.7 991-992 bp 1.0 77.0
trnK-UUU-rps16_p3 AAAGCCGAGTACTCTACCGT TCCTTGAAAAAGGGGCTCAG 50.0 50.0 58.2 57.7 931-932 bp 1.0 76.3
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT TCCTTGAAAAAGGGGCTCAG 50.0 50.0 58.2 57.7 930-931 bp 1.0 76.3
trnK-UUU-rps16_p5 TCAGTCGTGGTCTTCCAAAC TCCTTGAAAAAGGGGCTCAG 50.0 50.0 57.8 57.7 1016-1017 bp 1.0 75.8