Hotspot

rps16

Genome · 3533-4613 · 1081 bp

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Pi 0

MarkerSeek score 61.30

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: Genome
length_bp: 1081
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	CTGTAAGCTGGGCCCATTAG	ATGCGTCAGAAGGTTCGTAC	55.0	50.0	58.0	58.0	1171-1198 bp	1.0	81.6
rps16_p2	GCTGGGCCCATTAGATTCTC	ATGCGTCAGAAGGTTCGTAC	55.0	50.0	58.1	58.0	1165-1192 bp	1.0	81.1
rps16_p3	CTGTAAGCTGGGCCCATTAG	AGGAATGCGTCAGAAGGTTC	55.0	50.0	58.0	57.9	1175-1202 bp	1.0	80.9
rps16_p4	GCTGGGCCCATTAGATTCTC	AGGAATGCGTCAGAAGGTTC	55.0	50.0	58.1	57.9	1169-1196 bp	1.0	80.3
rps16_p5	AAGCTGGGCCCATTAGATTC	ATGCGTCAGAAGGTTCGTAC	50.0	50.0	57.6	58.0	1167-1194 bp	1.0	78.9