Hotspot

petN

Genome · 29215-29304 · 90 bp

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Pi 0.0111

MarkerSeek score 58.90

Primer available yes

Label petN

Feature Metadata

label_name: petN
feature_type: gene
region: Genome
length_bp: 90
variable_sites: 1
pi: 0.0111
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN_p1	AATATAGGGTGGGGGAAGCA	TTTTCCAAGCTTTGCCTTGC	50.0	45.0	57.8	58.0	257 bp	1.0	78.1
petN_p2	AATATAGGGTGGGGGAAGCA	CTCCTCACTGACAACGCATT	50.0	50.0	57.8	58.2	283 bp	1.0	76.9
petN_p3	AATATAGGGTGGGGGAAGCA	GTCTGCTCCTCACTGACAAC	50.0	55.0	57.8	58.2	288 bp	1.0	76.8
petN_p4	AATATAGGGTGGGGGAAGCA	TCCTCACTGACAACGCATTT	50.0	45.0	57.8	57.7	282 bp	1.0	76.5
petN_p5	ATATAGGGTGGGGGAAGCAC	TTTTCCAAGCTTTGCCTTGC	55.0	45.0	58.6	58.0	256 bp	1.0	75.5