Hotspot

petB

Genome · 70102-71564 · 1463 bp

Back to Onoclea

Pi 0.00137

MarkerSeek score 59.50

Primer available yes

Label petB

Feature Metadata

label_name: petB
feature_type: gene
region: Genome
length_bp: 1463
variable_sites: 2
pi: 0.00137
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB_p1	CTGGGGTTCGCAAATGGATA	TTATCACCCCCGACTCTTGT	50.0	50.0	57.9	58.1	1592-1593 bp	1.0	79.3
petB_p2	GGGGTTCGCAAATGGATACT	TTATCACCCCCGACTCTTGT	50.0	50.0	57.9	58.1	1590-1591 bp	1.0	79.3
petB_p3	CTGGGGTTCGCAAATGGATA	AAGTATTGGCGGCAACAGAA	50.0	45.0	57.9	58.1	1669-1670 bp	1.0	78.9
petB_p4	GGGGTTCGCAAATGGATACT	AAGTATTGGCGGCAACAGAA	50.0	45.0	57.9	58.1	1667-1668 bp	1.0	78.9
petB_p5	GGGAGTTAAATCGCGCAAAC	TTATCACCCCCGACTCTTGT	50.0	50.0	58.1	58.1	1553-1554 bp	1.0	78.8