Hotspot

ndhF

Genome · 104084-106324 · 2241 bp

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Pi 0.000446
MarkerSeek score 57.50
Primer available yes
Label ndhF

Feature Metadata

label_name
ndhF
feature_type
gene
region
Genome
length_bp
2241
variable_sites
1
pi
0.000446
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF_p1 AAAAGGAGCAAAAGGGGGAG CCGACTCCCAATTGGAAGAG 50.0 55.0 58.0 58.0 2393 bp 1.0 79.7
ndhF_p2 AAAAGGAGCAAAAGGGGGAG CGACTCCCAATTGGAAGAGG 50.0 55.0 58.0 58.0 2392 bp 1.0 79.7
ndhF_p3 GAGCAAAAGGGGGAGAGATG CCGACTCCCAATTGGAAGAG 55.0 55.0 58.0 58.0 2388 bp 1.0 79.4
ndhF_p4 GAGCAAAAGGGGGAGAGATG CGACTCCCAATTGGAAGAGG 55.0 55.0 58.0 58.0 2387 bp 1.0 79.4
ndhF_p5 GTCGGGAGAAATGAACACGA CGACTCCCAATTGGAAGAGG 50.0 55.0 57.9 58.0 2345 bp 1.0 79.2