Hotspot

ndhD

Genome · 110113-111621 · 1509 bp

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Pi 0.000663

MarkerSeek score 59.10

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: Genome
length_bp: 1509
variable_sites: 1
pi: 0.000663
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	GCGAGCTTTTCTTTTCCACC	ACTTCAGGATTGCGAAGTCC	50.0	50.0	57.9	57.9	1694-1695 bp	1.0	78.8
ndhD_p2	GGCGAGCTTTTCTTTTCCAC	ACTTCAGGATTGCGAAGTCC	50.0	50.0	57.9	57.9	1695-1696 bp	1.0	78.8
ndhD_p3	GCGAGCTTTTCTTTTCCACC	ATTGCGAAGTCCGAGTATGG	50.0	50.0	57.9	57.8	1686-1687 bp	1.0	77.8
ndhD_p4	GGCGAGCTTTTCTTTTCCAC	ATTGCGAAGTCCGAGTATGG	50.0	50.0	57.9	57.8	1687-1688 bp	1.0	77.8
ndhD_p5	GCGAGCTTTTCTTTTCCACC	GGTCGTTATAGTTGGCCCAC	50.0	55.0	57.9	58.3	1667-1668 bp	1.0	76.9