Hotspot

ndhB

Genome · 1-1207 · 1207 bp

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Pi 0

MarkerSeek score 40.00

Primer available yes

Label ndhB

Feature Metadata

label_name: ndhB
feature_type: gene
region: Genome
length_bp: 1207
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhB_p1	CGCTGTTGGTCGAACAATTC	AAATCCGTGTGGCAGAAAGT	50.0	45.0	58.0	58.0	963 bp	1.0	79.5
ndhB_p2	CGCTGTTGGTCGAACAATTC	AATCCGTGTGGCAGAAAGTT	50.0	45.0	58.0	58.0	962 bp	1.0	79.5
ndhB_p3	CGCTGTTGGTCGAACAATTC	GTGTGGCAGAAAGTTACCCA	50.0	50.0	58.0	58.0	957 bp	1.0	79.4
ndhB_p4	CGCTGTTGGTCGAACAATTC	TGTGGCAGAAAGTTACCCAC	50.0	50.0	58.0	58.0	956 bp	1.0	79.4
ndhB_p5	CGCTGTTGGTCGAACAATTC	TCCGTGTGGCAGAAAGTTAC	50.0	50.0	58.0	58.1	960 bp	1.0	78.6