Hotspot

matK

Genome · 1417-2916 · 1500 bp

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Pi 0

MarkerSeek score 40.00

Primer available yes

Label matK

Feature Metadata

label_name: matK
feature_type: gene
region: Genome
length_bp: 1500
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK_p1	TGAATTGCGTTGGAAAGGGA	CCCCCACCTGATTTGCATAA	45.0	50.0	58.0	57.8	1642 bp	1.0	78.6
matK_p2	AAAGGGAGAAGAGGCAGACT	CCCCCACCTGATTTGCATAA	50.0	50.0	58.0	57.8	1629 bp	1.0	78.4
matK_p3	AAGGGAGAAGAGGCAGACTT	CCCCCACCTGATTTGCATAA	50.0	50.0	58.0	57.8	1628 bp	1.0	78.4
matK_p4	GTGAATTGCGTTGGAAAGGG	CCCCCACCTGATTTGCATAA	50.0	50.0	57.9	57.8	1643 bp	1.0	78.2
matK_p5	CGTTGGAAAGGGAGAAGAGG	CCCCCACCTGATTTGCATAA	55.0	50.0	57.9	57.8	1635 bp	1.0	77.9