Hotspot

atpB

Genome · 49366-50847 · 1482 bp

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Pi 0.000675

MarkerSeek score 59.10

Primer available yes

Label atpB

Feature Metadata

label_name: atpB
feature_type: gene
region: Genome
length_bp: 1482
variable_sites: 1
pi: 0.000675
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB_p1	TGCCATTACGCGAAGATTCA	AGGAGGGGAGCGATTAACTT	45.0	50.0	58.0	58.1	1626 bp	1.0	79.0
atpB_p2	CCCAAACCTCGGAATTCCAA	AGGAGGGGAGCGATTAACTT	50.0	50.0	58.1	58.1	1660 bp	1.0	78.4
atpB_p3	TGCCATTACGCGAAGATTCA	CCACTCGACGATGAAGTACC	45.0	55.0	58.0	57.8	1591 bp	1.0	78.4
atpB_p4	AGTATACCAACCTGACCGCT	AGGAGGGGAGCGATTAACTT	50.0	50.0	58.1	58.1	1700 bp	1.0	78.0
atpB_p5	ATACCAACCTGACCGCTACT	AGGAGGGGAGCGATTAACTT	50.0	50.0	58.1	58.1	1697 bp	1.0	78.0