Hotspot

trnR-ACG-trnT-UGU

Genome · 145750-146467 · 718 bp

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Pi 0.00279

MarkerSeek score 60.40

Primer available yes

Label trnR-ACG-trnT-UGU

Feature Metadata

label_name: trnR-ACG-trnT-UGU
feature_type: igs
region: Genome
length_bp: 718
variable_sites: 2
pi: 0.00279
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-ACG-trnT-UGU_p1	GCGAATATGGATCCAGTGGG	GTGGTACGGAGGTTCGAATC	55.0	55.0	58.2	58.1	850-904 bp	1.0	78.0
trnR-ACG-trnT-UGU_p2	GCGAATATGGATCCAGTGGG	TCGAAATGTAGTACCGCGTG	55.0	50.0	58.2	58.1	867-921 bp	1.0	77.9
trnR-ACG-trnT-UGU_p3	GCGAATATGGATCCAGTGGG	ACTCGAAATGTAGTACCGCG	55.0	50.0	58.2	57.8	869-923 bp	1.0	77.2
trnR-ACG-trnT-UGU_p4	GCGAATATGGATCCAGTGGG	CTCGAAATGTAGTACCGCGT	55.0	50.0	58.2	57.8	868-922 bp	1.0	77.2
trnR-ACG-trnT-UGU_p5	GCGAATATGGATCCAGTGGG	GGAGGTTCGAATCCTATGCG	55.0	55.0	58.2	58.2	843-897 bp	1.0	77.0