Hotspot

trnT-UGU-trnR-ACG

Genome · 83500-84217 · 718 bp

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Pi 0.00279
MarkerSeek score 60.40
Primer available yes
Label trnT-UGU-trnR-ACG

Feature Metadata

label_name
trnT-UGU-trnR-ACG
feature_type
igs
region
Genome
length_bp
718
variable_sites
2
pi
0.00279
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-UGU-trnR-ACG_p1 GTGGTACGGAGGTTCGAATC GCGAATATGGATCCAGTGGG 55.0 55.0 58.1 58.2 850-904 bp 1.0 78.0
trnT-UGU-trnR-ACG_p2 TCGAAATGTAGTACCGCGTG GCGAATATGGATCCAGTGGG 50.0 55.0 58.1 58.2 867-921 bp 1.0 77.9
trnT-UGU-trnR-ACG_p3 ACTCGAAATGTAGTACCGCG GCGAATATGGATCCAGTGGG 50.0 55.0 57.8 58.2 869-923 bp 1.0 77.2
trnT-UGU-trnR-ACG_p4 CTCGAAATGTAGTACCGCGT GCGAATATGGATCCAGTGGG 50.0 55.0 57.8 58.2 868-922 bp 1.0 77.2
trnT-UGU-trnR-ACG_p5 GGAGGTTCGAATCCTATGCG GCGAATATGGATCCAGTGGG 55.0 55.0 58.2 58.2 843-897 bp 1.0 77.0