Hotspot

psbE-petL

Genome · 60321-61079 · 759 bp

Back to Onoclea

Pi 0.00132

MarkerSeek score 59.50

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: Genome
length_bp: 759
variable_sites: 1
pi: 0.00132
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	GCGTTCTCCTGTATTCCCAG	GTCGAAAAGGCGAAGTAGCT	55.0	50.0	58.1	58.3	818 bp	1.0	77.3
psbE-petL_p2	GCGTTCTCCTGTATTCCCAG	TCGAAAAGGCGAAGTAGCTT	55.0	45.0	58.1	57.5	817 bp	1.0	76.0
psbE-petL_p3	GTAATGCTGTGGATGACCCA	GTCGAAAAGGCGAAGTAGCT	50.0	50.0	57.6	58.3	871 bp	1.0	74.4
psbE-petL_p4	GTAATGCTGTGGATGACCCA	TCGAAAAGGCGAAGTAGCTT	50.0	45.0	57.6	57.5	870 bp	1.0	73.1
psbE-petL_p5	AAGGGGCGTTCTCCTGTATT	GTCGAAAAGGCGAAGTAGCT	50.0	50.0	58.7	58.3	823 bp	1.0	72.1