Hotspot

psbM-petN

Genome · 28349-29214 · 866 bp

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Pi 0

MarkerSeek score 29.30

Primer available yes

Label psbM-petN

Feature Metadata

label_name: psbM-petN
feature_type: igs
region: Genome
length_bp: 866
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 29.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-petN_p1	TCTCTACATTCAAACGGCCG	TCTCACTTGTAGTTCGGGGA	50.0	50.0	58.0	57.7	935 bp	1.0	77.6
psbM-petN_p2	ACCGCACTTTTCATCCCAAT	TCTCACTTGTAGTTCGGGGA	45.0	50.0	58.1	57.7	976 bp	1.0	77.0
psbM-petN_p3	CATCCCAATCCCAACAGCTT	TCTCACTTGTAGTTCGGGGA	50.0	50.0	58.1	57.7	965 bp	1.0	76.5
psbM-petN_p4	TTCATCCCAATCCCAACAGC	TCTCACTTGTAGTTCGGGGA	50.0	50.0	58.1	57.7	967 bp	1.0	76.4
psbM-petN_p5	TCTCTACATTCAAACGGCCG	TTGTAGTTCGGGGAAGAAGC	50.0	50.0	58.0	57.5	929 bp	1.0	76.2