Hotspot

atpF-atpH

Genome · 12967-13319 · 353 bp

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Pi 0.0142

MarkerSeek score 53.60

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: Genome
length_bp: 353
variable_sites: 5
pi: 0.0142
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	GGAGCAGCGATCTCACTTAC	GGATCAGTTGTAGCTCCAGC	55.0	55.0	58.2	58.1	430-444 bp	1.0	79.4
atpF-atpH_p2	CGGCAAGAGTCCAATCACTT	GGATCAGTTGTAGCTCCAGC	50.0	55.0	58.2	58.1	453-467 bp	1.0	79.1
atpF-atpH_p3	AATACTTGCGGCAAGAGTCC	GGATCAGTTGTAGCTCCAGC	50.0	55.0	58.3	58.1	461-475 bp	1.0	78.5
atpF-atpH_p4	AGCAGCGATCTCACTTACAC	GGATCAGTTGTAGCTCCAGC	50.0	55.0	57.7	58.1	428-442 bp	1.0	78.4
atpF-atpH_p5	GCAGCGATCTCACTTACACT	GGATCAGTTGTAGCTCCAGC	50.0	55.0	57.7	58.1	427-441 bp	1.0	78.4