primer_id label_name rank fwd_seq rev_seq fwd_len rev_len fwd_gc rev_gc fwd_tm rev_tm fwd_self_any_th rev_self_any_th primer3_penalty target_start target_end amplicon_size cross_species_success_rate primer_score ndhB_p1 ndhB 1 CGCTGTTGGTCGAACAATTC AAATCCGTGTGGCAGAAAGT 20 20 50.0 45.0 58.0 58.0 20.6 0.0 0.068272 401 1200 963-963 1.000000 79.5 ndhB_p2 ndhB 2 CGCTGTTGGTCGAACAATTC AATCCGTGTGGCAGAAAGTT 20 20 50.0 45.0 58.0 58.0 20.6 0.0 0.068272 401 1200 962-962 1.000000 79.5 ndhB_p3 ndhB 3 CGCTGTTGGTCGAACAATTC GTGTGGCAGAAAGTTACCCA 20 20 50.0 50.0 58.0 58.0 20.6 0.0 0.073869 401 1200 957-957 1.000000 79.4 ndhB_p4 ndhB 4 CGCTGTTGGTCGAACAATTC TGTGGCAGAAAGTTACCCAC 20 20 50.0 50.0 58.0 58.0 20.6 0.0 0.073869 401 1200 956-956 1.000000 79.4 ndhB_p5 ndhB 5 CGCTGTTGGTCGAACAATTC TCCGTGTGGCAGAAAGTTAC 20 20 50.0 50.0 58.0 58.1 20.6 0.0 0.175831 401 1200 960-960 1.000000 78.6 matK_p1 matK 1 TGAATTGCGTTGGAAAGGGA CCCCCACCTGATTTGCATAA 20 20 45.0 50.0 58.0 57.8 0.0 0.0 0.169346 1417 2916 1642-1642 1.000000 78.6 matK_p2 matK 2 AAAGGGAGAAGAGGCAGACT CCCCCACCTGATTTGCATAA 20 20 50.0 50.0 58.0 57.8 0.0 0.0 0.195025 1417 2916 1629-1629 1.000000 78.4 matK_p3 matK 3 AAGGGAGAAGAGGCAGACTT CCCCCACCTGATTTGCATAA 20 20 50.0 50.0 58.0 57.8 0.0 0.0 0.195025 1417 2916 1628-1628 1.000000 78.4 matK_p4 matK 4 GTGAATTGCGTTGGAAAGGG CCCCCACCTGATTTGCATAA 20 20 50.0 50.0 57.9 57.8 0.0 0.0 0.222213 1417 2916 1643-1643 1.000000 78.2 matK_p5 matK 5 CGTTGGAAAGGGAGAAGAGG CCCCCACCTGATTTGCATAA 20 20 55.0 50.0 57.9 57.8 0.0 0.0 0.258244 1417 2916 1635-1635 1.000000 77.9 matK-rps16_p1 matK-rps16 1 CGCTAAGTCCTTCTTCCTGG ATTTTGAGACGGGCGAAGTT 20 20 55.0 45.0 57.7 58.1 0.0 0.0 0.409368 2917 3532 743-811 1.000000 77.1 matK-rps16_p2 matK-rps16 2 CGCTAAGTCCTTCTTCCTGG GGGCGCAATCAACAAAAACT 20 20 55.0 45.0 57.7 58.1 0.0 10.8 0.437012 2917 3532 772-840 1.000000 76.9 matK-rps16_p3 matK-rps16 3 CGCTAAGTCCTTCTTCCTGG TGAACAAGTCGGGATCAACC 20 20 55.0 50.0 57.7 57.8 0.0 0.0 0.480489 2917 3532 720-788 1.000000 76.6 matK-rps16_p4 matK-rps16 4 CGCTAAGTCCTTCTTCCTGG TAAGAAAGGGGCGCAATCAA 20 20 55.0 45.0 57.7 57.8 0.0 10.8 0.509709 2917 3532 780-848 1.000000 76.3 matK-rps16_p5 matK-rps16 5 CGCTAAGTCCTTCTTCCTGG TTAAGAAAGGGGCGCAATCA 20 20 55.0 45.0 57.7 57.8 0.0 10.8 0.509709 2917 3532 781-849 1.000000 76.3 rps16_p1 rps16 1 CTGTAAGCTGGGCCCATTAG ATGCGTCAGAAGGTTCGTAC 20 20 55.0 50.0 58.0 58.0 28.8 0.0 0.034958 3533 4613 1171-1198 1.000000 81.6 rps16_p2 rps16 2 GCTGGGCCCATTAGATTCTC ATGCGTCAGAAGGTTCGTAC 20 20 55.0 50.0 58.1 58.0 28.8 0.0 0.104892 3533 4613 1165-1192 1.000000 81.1 rps16_p3 rps16 3 CTGTAAGCTGGGCCCATTAG AGGAATGCGTCAGAAGGTTC 20 20 55.0 50.0 58.0 57.9 28.8 0.0 0.127733 3533 4613 1175-1202 1.000000 80.9 rps16_p4 rps16 4 GCTGGGCCCATTAGATTCTC AGGAATGCGTCAGAAGGTTC 20 20 55.0 50.0 58.1 57.9 28.8 0.0 0.197667 3533 4613 1169-1196 1.000000 80.3 rps16_p5 rps16 5 AAGCTGGGCCCATTAGATTC ATGCGTCAGAAGGTTCGTAC 20 20 50.0 50.0 57.6 58.0 28.8 0.0 0.379488 3533 4613 1167-1194 1.000000 78.9 trnG-UCC_p1 trnG-UCC 1 GGGGGACTCCTCCTATAACC CCCAAATCGTGAACAAACGG 20 20 60.0 50.0 58.0 58.0 6.8 0.0 0.071986 8785 9774 1175-1175 1.000000 79.4 trnG-UCC_p2 trnG-UCC 2 GGGGGACTCCTCCTATAACC CCCCAAATCGTGAACAAACG 20 20 60.0 50.0 58.0 58.0 6.8 0.0 0.071986 8785 9774 1176-1176 1.000000 79.4 trnG-UCC_p3 trnG-UCC 3 GGGGGACTCCTCCTATAACC TTGTTCACGACACAATCCGT 20 20 60.0 45.0 58.0 58.1 6.8 0.0 0.084420 8785 9774 1145-1145 1.000000 79.3 trnG-UCC_p4 trnG-UCC 4 GGGGGACTCCTCCTATAACC ACTGGGGGCTCCAATATTCT 20 20 60.0 50.0 58.0 58.1 6.8 7.9 0.114236 8785 9774 1106-1106 1.000000 79.1 trnG-UCC_p5 trnG-UCC 5 GGGGGACTCCTCCTATAACC TTAGCCCCCAAATCGTGAAC 20 20 60.0 50.0 58.0 58.2 6.8 0.0 0.198118 8785 9774 1181-1181 1.000000 78.4 atpF-atpH_p1 atpF-atpH 1 GGAGCAGCGATCTCACTTAC GGATCAGTTGTAGCTCCAGC 20 20 55.0 55.0 58.2 58.1 0.0 0.0 0.206714 12967 13319 430-444 1.000000 79.4 atpF-atpH_p2 atpF-atpH 2 CGGCAAGAGTCCAATCACTT GGATCAGTTGTAGCTCCAGC 20 20 50.0 55.0 58.2 58.1 0.0 0.0 0.239742 12967 13319 453-467 1.000000 79.1 atpF-atpH_p3 atpF-atpH 3 AATACTTGCGGCAAGAGTCC GGATCAGTTGTAGCTCCAGC 20 20 50.0 55.0 58.3 58.1 40.4 0.0 0.314188 12967 13319 461-475 1.000000 78.5 atpF-atpH_p4 atpF-atpH 4 AGCAGCGATCTCACTTACAC GGATCAGTTGTAGCTCCAGC 20 20 50.0 55.0 57.7 58.1 0.0 0.0 0.339082 12967 13319 428-442 1.000000 78.4 atpF-atpH_p5 atpF-atpH 5 GCAGCGATCTCACTTACACT GGATCAGTTGTAGCTCCAGC 20 20 50.0 55.0 57.7 58.1 0.0 0.0 0.339082 12967 13319 427-441 1.000000 78.4 atpH_p1 atpH 1 CTACTCCCCGCTACAAACAG CGTTGCGCCGATTCTAGTTA 20 20 55.0 50.0 57.7 58.4 0.0 4.2 0.743027 13320 13565 343-343 1.000000 74.3 atpH_p2 atpH 2 CTACTCCCCGCTACAAACAG TAGAAAGTCTTCGTTGCGCC 20 20 55.0 50.0 57.7 58.6 0.0 4.2 0.869841 13320 13565 354-354 1.000000 73.3 atpH_p3 atpH 3 CTACTCCCCGCTACAAACAG CTAGAAAGTCTTCGTTGCGC 20 20 55.0 50.0 57.7 57.2 0.0 0.0 1.066463 13320 13565 355-355 1.000000 71.7 atpH_p4 atpH 4 CTACTCCCCGCTACAAACAG TTGCGCCGATTCTAGTTAGT 20 20 55.0 45.0 57.7 57.0 0.0 4.2 1.248719 13320 13565 341-341 1.000000 70.2 atpH_p5 atpH 5 GCTACTCCCCGCTACAAAC CGTTGCGCCGATTCTAGTTA 19 20 57.9 50.0 57.9 58.4 0.0 4.2 1.508867 13320 13565 344-344 1.000000 68.2 rpoC1_p1 rpoC1 1 TCATTATAGAAACCTCACCTTGA TGGAGAGGGAGAGTGAAAACA 23 21 34.8 47.6 54.8 58.3 0.0 0.0 7.520763 20521 23322 2995-2998 1.000000 40.1 rpoC1_p2 rpoC1 2 TCATTATAGAAACCTCACCTTGA GGAGAGGGAGAGTGAAAACA 23 20 34.8 50.0 54.8 56.5 0.0 0.0 7.735104 20521 23322 2994-2997 1.000000 40.1 rpoC1_p3 rpoC1 3 TCATTATAGAAACCTCACCTTGA TGGAGAGGGAGAGTGAAAAC 23 20 34.8 50.0 54.8 56.5 0.0 0.0 7.735104 20521 23322 2995-2998 1.000000 40.1 rpoC1_p4 rpoC1 4 TCATTATAGAAACCTCACCTTGAT TGGAGAGGGAGAGTGAAAACA 24 21 33.3 47.6 55.3 58.3 0.0 0.0 7.980878 20521 23322 2995-2998 1.000000 40.1 rpoC1_p5 rpoC1 5 TCATTATAGAAACCTCACCTTGA TTGGAGAGGGAGAGTGAAAAC 23 21 34.8 47.6 54.8 57.2 0.0 0.0 7.981998 20521 23322 2996-2999 1.000000 40.1 psbM-petN_p1 psbM-petN 1 TCTCTACATTCAAACGGCCG TCTCACTTGTAGTTCGGGGA 20 20 50.0 50.0 58.0 57.7 26.9 0.0 0.302281 28349 29214 935-935 1.000000 77.6 psbM-petN_p2 psbM-petN 2 ACCGCACTTTTCATCCCAAT TCTCACTTGTAGTTCGGGGA 20 20 45.0 50.0 58.1 57.7 0.0 0.0 0.374343 28349 29214 976-976 1.000000 77.0 psbM-petN_p3 psbM-petN 3 CATCCCAATCCCAACAGCTT TCTCACTTGTAGTTCGGGGA 20 20 50.0 50.0 58.1 57.7 0.0 0.0 0.441069 28349 29214 965-965 1.000000 76.5 psbM-petN_p4 psbM-petN 4 TTCATCCCAATCCCAACAGC TCTCACTTGTAGTTCGGGGA 20 20 50.0 50.0 58.1 57.7 0.0 0.0 0.446394 28349 29214 967-967 1.000000 76.4 psbM-petN_p5 psbM-petN 5 TCTCTACATTCAAACGGCCG TTGTAGTTCGGGGAAGAAGC 20 20 50.0 50.0 58.0 57.5 26.9 0.0 0.472148 28349 29214 929-929 1.000000 76.2 petN_p1 petN 1 AATATAGGGTGGGGGAAGCA TTTTCCAAGCTTTGCCTTGC 20 20 50.0 45.0 57.8 58.0 0.0 0.0 0.270268 29215 29304 257-257 1.000000 78.1 petN_p2 petN 2 AATATAGGGTGGGGGAAGCA CTCCTCACTGACAACGCATT 20 20 50.0 50.0 57.8 58.2 0.0 0.0 0.421656 29215 29304 283-283 1.000000 76.9 petN_p3 petN 3 AATATAGGGTGGGGGAAGCA GTCTGCTCCTCACTGACAAC 20 20 50.0 55.0 57.8 58.2 0.0 0.0 0.432955 29215 29304 288-288 1.000000 76.8 petN_p4 petN 4 AATATAGGGTGGGGGAAGCA TCCTCACTGACAACGCATTT 20 20 50.0 45.0 57.8 57.7 0.0 0.0 0.477007 29215 29304 282-282 1.000000 76.5 petN_p5 petN 5 ATATAGGGTGGGGGAAGCAC TTTTCCAAGCTTTGCCTTGC 20 20 55.0 45.0 58.6 58.0 0.0 0.0 0.601792 29215 29304 256-256 1.000000 75.5 atpB_p1 atpB 1 TGCCATTACGCGAAGATTCA AGGAGGGGAGCGATTAACTT 20 20 45.0 50.0 58.0 58.1 15.9 0.0 0.136197 49366 50847 1626-1626 1.000000 79.0 atpB_p2 atpB 2 CCCAAACCTCGGAATTCCAA AGGAGGGGAGCGATTAACTT 20 20 50.0 50.0 58.1 58.1 24.1 0.0 0.201187 49366 50847 1660-1660 1.000000 78.4 atpB_p3 atpB 3 TGCCATTACGCGAAGATTCA CCACTCGACGATGAAGTACC 20 20 45.0 55.0 58.0 57.8 15.9 0.0 0.207463 49366 50847 1591-1591 1.000000 78.4 atpB_p4 atpB 4 AGTATACCAACCTGACCGCT AGGAGGGGAGCGATTAACTT 20 20 50.0 50.0 58.1 58.1 0.0 0.0 0.257077 49366 50847 1700-1700 1.000000 78.0 atpB_p5 atpB 5 ATACCAACCTGACCGCTACT AGGAGGGGAGCGATTAACTT 20 20 50.0 50.0 58.1 58.1 0.0 0.0 0.257077 49366 50847 1697-1697 1.000000 78.0 psbE-petL_p1 psbE-petL 1 GCGTTCTCCTGTATTCCCAG GTCGAAAAGGCGAAGTAGCT 20 20 55.0 50.0 58.1 58.3 0.0 0.0 0.347469 60321 61079 818-818 1.000000 77.3 psbE-petL_p2 psbE-petL 2 GCGTTCTCCTGTATTCCCAG TCGAAAAGGCGAAGTAGCTT 20 20 55.0 45.0 58.1 57.5 0.0 0.0 0.509284 60321 61079 817-817 1.000000 76.0 psbE-petL_p3 psbE-petL 3 GTAATGCTGTGGATGACCCA GTCGAAAAGGCGAAGTAGCT 20 20 50.0 50.0 57.6 58.3 0.0 0.0 0.707162 60321 61079 871-871 1.000000 74.4 psbE-petL_p4 psbE-petL 4 GTAATGCTGTGGATGACCCA TCGAAAAGGCGAAGTAGCTT 20 20 50.0 45.0 57.6 57.5 0.0 0.0 0.868977 60321 61079 870-870 1.000000 73.1 psbE-petL_p5 psbE-petL 5 AAGGGGCGTTCTCCTGTATT GTCGAAAAGGCGAAGTAGCT 20 20 50.0 50.0 58.7 58.3 0.0 0.0 1.002546 60321 61079 823-823 1.000000 72.1 petB_p1 petB 1 CTGGGGTTCGCAAATGGATA TTATCACCCCCGACTCTTGT 20 20 50.0 50.0 57.9 58.1 0.0 0.0 0.104350 70102 71564 1592-1593 1.000000 79.3 petB_p2 petB 2 GGGGTTCGCAAATGGATACT TTATCACCCCCGACTCTTGT 20 20 50.0 50.0 57.9 58.1 0.0 0.0 0.105798 70102 71564 1590-1591 1.000000 79.3 petB_p3 petB 3 CTGGGGTTCGCAAATGGATA AAGTATTGGCGGCAACAGAA 20 20 50.0 45.0 57.9 58.1 0.0 28.0 0.147048 70102 71564 1669-1670 1.000000 78.9 petB_p4 petB 4 GGGGTTCGCAAATGGATACT AAGTATTGGCGGCAACAGAA 20 20 50.0 45.0 57.9 58.1 0.0 28.0 0.148495 70102 71564 1667-1668 1.000000 78.9 petB_p5 petB 5 GGGAGTTAAATCGCGCAAAC TTATCACCCCCGACTCTTGT 20 20 50.0 50.0 58.1 58.1 8.7 0.0 0.161837 70102 71564 1553-1554 1.000000 78.8 trnT-UGU-trnR-ACG_p1 trnT-UGU-trnR-ACG 1 GTGGTACGGAGGTTCGAATC GCGAATATGGATCCAGTGGG 20 20 55.0 55.0 58.1 58.2 0.0 21.3 0.267892 83500 84217 850-904 1.000000 78.0 trnT-UGU-trnR-ACG_p2 trnT-UGU-trnR-ACG 2 TCGAAATGTAGTACCGCGTG GCGAATATGGATCCAGTGGG 20 20 50.0 55.0 58.1 58.2 4.9 21.3 0.287548 83500 84217 867-921 1.000000 77.9 trnT-UGU-trnR-ACG_p3 trnT-UGU-trnR-ACG 3 ACTCGAAATGTAGTACCGCG GCGAATATGGATCCAGTGGG 20 20 50.0 55.0 57.8 58.2 0.0 21.3 0.370871 83500 84217 869-923 1.000000 77.2 trnT-UGU-trnR-ACG_p4 trnT-UGU-trnR-ACG 4 CTCGAAATGTAGTACCGCGT GCGAATATGGATCCAGTGGG 20 20 50.0 55.0 57.8 58.2 4.9 21.3 0.370871 83500 84217 868-922 1.000000 77.2 trnT-UGU-trnR-ACG_p5 trnT-UGU-trnR-ACG 5 GGAGGTTCGAATCCTATGCG GCGAATATGGATCCAGTGGG 20 20 55.0 55.0 58.2 58.2 16.2 21.3 0.401962 83500 84217 843-897 1.000000 77.0 ndhF_p1 ndhF 1 AAAAGGAGCAAAAGGGGGAG CCGACTCCCAATTGGAAGAG 20 20 50.0 55.0 58.0 58.0 0.0 26.6 0.045295 104084 106324 2393-2393 1.000000 79.7 ndhF_p2 ndhF 2 AAAAGGAGCAAAAGGGGGAG CGACTCCCAATTGGAAGAGG 20 20 50.0 55.0 58.0 58.0 0.0 26.6 0.045295 104084 106324 2392-2392 1.000000 79.7 ndhF_p3 ndhF 3 GAGCAAAAGGGGGAGAGATG CCGACTCCCAATTGGAAGAG 20 20 55.0 55.0 58.0 58.0 0.0 26.6 0.079727 104084 106324 2388-2388 1.000000 79.4 ndhF_p4 ndhF 4 GAGCAAAAGGGGGAGAGATG CGACTCCCAATTGGAAGAGG 20 20 55.0 55.0 58.0 58.0 0.0 26.6 0.079727 104084 106324 2387-2387 1.000000 79.4 ndhF_p5 ndhF 5 GTCGGGAGAAATGAACACGA CGACTCCCAATTGGAAGAGG 20 20 50.0 55.0 57.9 58.0 0.0 26.6 0.104385 104084 106324 2345-2345 1.000000 79.2 ndhD_p1 ndhD 1 GCGAGCTTTTCTTTTCCACC ACTTCAGGATTGCGAAGTCC 20 20 50.0 50.0 57.9 57.9 0.0 0.0 0.152483 110113 111621 1694-1695 1.000000 78.8 ndhD_p2 ndhD 2 GGCGAGCTTTTCTTTTCCAC ACTTCAGGATTGCGAAGTCC 20 20 50.0 50.0 57.9 57.9 0.0 0.0 0.152483 110113 111621 1695-1696 1.000000 78.8 ndhD_p3 ndhD 3 GCGAGCTTTTCTTTTCCACC ATTGCGAAGTCCGAGTATGG 20 20 50.0 50.0 57.9 57.8 0.0 0.0 0.276947 110113 111621 1686-1687 1.000000 77.8 ndhD_p4 ndhD 4 GGCGAGCTTTTCTTTTCCAC ATTGCGAAGTCCGAGTATGG 20 20 50.0 50.0 57.9 57.8 0.0 0.0 0.276947 110113 111621 1687-1688 1.000000 77.8 ndhD_p5 ndhD 5 GCGAGCTTTTCTTTTCCACC GGTCGTTATAGTTGGCCCAC 20 20 50.0 55.0 57.9 58.3 0.0 0.0 0.391712 110113 111621 1667-1668 1.000000 76.9 ycf1_p1 ycf1 1 AACATGCGGCCAAAAGTTTT TTGAGCAGCTAACAGGTTCG 20 20 40.0 50.0 57.7 58.2 2.2 0.0 0.528201 120974 121093 268-268 1.000000 76.1 ycf1_p2 ycf1 2 AACATGCGGCCAAAAGTTTT GGGGCCGAAAAATCGTTTAC 20 20 40.0 50.0 57.7 57.7 2.2 0.0 0.591156 120974 121093 248-248 1.000000 75.6 ycf1_p3 ycf1 3 CAACATGCGGCCAAAAGTTT TTGAGCAGCTAACAGGTTCG 20 20 45.0 50.0 58.4 58.2 2.2 0.0 0.623417 120974 121093 269-269 1.000000 75.3 ycf1_p4 ycf1 4 AACATGCGGCCAAAAGTTTT CTACCCGTTGAGCAGCTAAC 20 20 40.0 55.0 57.7 58.4 2.2 0.0 0.682391 120974 121093 275-275 1.000000 74.8 ycf1_p5 ycf1 5 CAACATGCGGCCAAAAGTTT GGGGCCGAAAAATCGTTTAC 20 20 45.0 50.0 58.4 57.7 2.2 0.0 0.686373 120974 121093 249-249 1.000000 74.8 trnR-ACG-trnT-UGU_p1 trnR-ACG-trnT-UGU 1 GCGAATATGGATCCAGTGGG GTGGTACGGAGGTTCGAATC 20 20 55.0 55.0 58.2 58.1 21.3 0.0 0.267892 145750 146467 850-904 1.000000 78.0 trnR-ACG-trnT-UGU_p2 trnR-ACG-trnT-UGU 2 GCGAATATGGATCCAGTGGG TCGAAATGTAGTACCGCGTG 20 20 55.0 50.0 58.2 58.1 21.3 4.9 0.287548 145750 146467 867-921 1.000000 77.9 trnR-ACG-trnT-UGU_p3 trnR-ACG-trnT-UGU 3 GCGAATATGGATCCAGTGGG ACTCGAAATGTAGTACCGCG 20 20 55.0 50.0 58.2 57.8 21.3 0.0 0.370871 145750 146467 869-923 1.000000 77.2 trnR-ACG-trnT-UGU_p4 trnR-ACG-trnT-UGU 4 GCGAATATGGATCCAGTGGG CTCGAAATGTAGTACCGCGT 20 20 55.0 50.0 58.2 57.8 21.3 4.9 0.370871 145750 146467 868-922 1.000000 77.2 trnR-ACG-trnT-UGU_p5 trnR-ACG-trnT-UGU 5 GCGAATATGGATCCAGTGGG GGAGGTTCGAATCCTATGCG 20 20 55.0 55.0 58.2 58.2 21.3 16.2 0.401962 145750 146467 843-897 1.000000 77.0