Hotspot

tRNA-Glu-tRNA-Thr

Genome · 113600-114063 · 464 bp

Back to Onobrychis

Pi 0.349

MarkerSeek score 68.30

Primer available yes

Label tRNA-Glu-tRNA-Thr

Feature Metadata

label_name: tRNA-Glu-tRNA-Thr
feature_type: igs
region: Genome
length_bp: 464
variable_sites: 103
pi: 0.349
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.39
markerseek_score: 68.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-Glu-tRNA-Thr_p1	ATCAGCAAATTTTTGGGCCG	GAACCGATGACTTACGCCTT	45.0	50.0	57.9	58.0	767-768 bp	0.5	63.2
tRNA-Glu-tRNA-Thr_p2	ATCAGCAAATTTTTGGGCCG	AGAATAAGCCCTTCTGCGTG	45.0	50.0	57.9	58.0	1338-1344 bp	0.5	63.0
tRNA-Glu-tRNA-Thr_p3	CGTTGCCTCCTTGAAAGAGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.8	58.0	572-573 bp	0.5	62.6
tRNA-Glu-tRNA-Thr_p4	GATCGACACAGATTACGCGT	GAACCGATGACTTACGCCTT	50.0	50.0	58.2	58.0	1024-1025 bp	0.5	62.5
tRNA-Glu-tRNA-Thr_p5	TGATCGACACAGATTACGCG	GAACCGATGACTTACGCCTT	50.0	50.0	58.2	58.0	1025-1026 bp	0.5	62.5