Hotspot

tRNA-Val-tRNA-Met

Genome · 49730-55572 · 5843 bp

Back to Onobrychis

Pi 0.283

MarkerSeek score 75.30

Primer available yes

Label tRNA-Val-tRNA-Met

Feature Metadata

label_name: tRNA-Val-tRNA-Met
feature_type: igs
region: Genome
length_bp: 5843
variable_sites: 2492
pi: 0.283
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 75.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-Val-tRNA-Met_p1	CCCTAGATGCTGTCGGAGTA	CTATTTGCCTCCGCCATTCT	55.0	50.0	58.0	58.0	572 bp	0.5	59.6
tRNA-Val-tRNA-Met_p2	CCCTAGATGCTGTCGGAGTA	CTCTATTTGCCTCCGCCATT	55.0	50.0	58.0	58.0	574 bp	0.5	59.6
tRNA-Val-tRNA-Met_p3	CCCTAGATGCTGTCGGAGTA	TCTATTTGCCTCCGCCATTC	55.0	50.0	58.0	58.0	573 bp	0.5	59.6
tRNA-Val-tRNA-Met_p4	AGGATTTACCCGGTGTGAGA	CTATTTGCCTCCGCCATTCT	50.0	50.0	58.1	58.0	611 bp	0.5	59.4
tRNA-Val-tRNA-Met_p5	AGGATTTACCCGGTGTGAGA	CTCTATTTGCCTCCGCCATT	50.0	50.0	58.1	58.0	613 bp	0.5	59.4