Hotspot

tRNA-Lys-tRNA-His

Genome · 19777-21283 · 1507 bp

Back to Onobrychis

Pi 0.293

MarkerSeek score 72.70

Primer available yes

Label tRNA-Lys-tRNA-His

Feature Metadata

label_name: tRNA-Lys-tRNA-His
feature_type: igs
region: Genome
length_bp: 1507
variable_sites: 665
pi: 0.293
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 72.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-Lys-tRNA-His_p1	GAAAGCCGTGTGCAATGAAA	ATGCCACTTTTTCGGTGAGT	45.0	45.0	57.9	58.0	2329 bp	0.5	58.9
tRNA-Lys-tRNA-His_p2	TGCAATGAAAAATGCACGCA	ATGCCACTTTTTCGGTGAGT	40.0	45.0	58.1	58.0	2319 bp	0.5	58.8
tRNA-Lys-tRNA-His_p3	ACAAAAGTAGTGTACGCGCT	ATGCCACTTTTTCGGTGAGT	45.0	45.0	57.9	58.0	2641 bp	0.5	58.8
tRNA-Lys-tRNA-His_p4	TGGGCCAATTCATCCGATTT	ATGCCACTTTTTCGGTGAGT	45.0	45.0	57.8	58.0	2795 bp	0.5	58.6
tRNA-Lys-tRNA-His_p5	CACAAAAGTAGTGTACGCGC	ATGCCACTTTTTCGGTGAGT	50.0	45.0	57.8	58.0	2642 bp	0.5	58.3