Hotspot

tRNA-Lys-tRNA-Lys

Genome · 17280-19747 · 2468 bp

Back to Onobrychis

Pi 0.27

MarkerSeek score 78.60

Primer available yes

Label tRNA-Lys-tRNA-Lys

Feature Metadata

label_name: tRNA-Lys-tRNA-Lys
feature_type: igs
region: Genome
length_bp: 2468
variable_sites: 1005
pi: 0.27
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 78.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-Lys-tRNA-Lys_p1	TCAATGGTAGAGTACTCGGCT	ACTCCCAAGCACAGGAATTC	47.6	50.0	58.0	57.8	2773-2774 bp	0.5	50.3
tRNA-Lys-tRNA-Lys_p2	TCAATGGTAGAGTACTCGGCT	GACTCCCAAGCACAGGAATT	47.6	50.0	58.0	57.8	2774-2775 bp	0.5	50.3
tRNA-Lys-tRNA-Lys_p3	TCAATGGTAGAGTACTCGGCT	ATATCTACTGGAGGGGCAGC	47.6	55.0	58.0	58.3	2989-2990 bp	0.5	49.1
tRNA-Lys-tRNA-Lys_p4	AGGGTTGCTAACTCAATGGT	ACTCCCAAGCACAGGAATTC	45.0	50.0	56.7	57.8	2785-2786 bp	0.5	48.2
tRNA-Lys-tRNA-Lys_p5	AGGGTTGCTAACTCAATGGT	GACTCCCAAGCACAGGAATT	45.0	50.0	56.7	57.8	2786-2787 bp	0.5	48.2