Hotspot

tRNA-Val-tRNA-Val

Genome · 11665-12244 · 580 bp

Back to Onobrychis

Pi 0.305

MarkerSeek score 81.50

Primer available yes

Label tRNA-Val-tRNA-Val

Feature Metadata

label_name: tRNA-Val-tRNA-Val
feature_type: igs
region: Genome
length_bp: 580
variable_sites: 266
pi: 0.305
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 81.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-Val-tRNA-Val_p1	GAAATTGTAACCACGCACCG	TGCAGAGAAGGGTAGTGACA	50.0	50.0	58.0	58.0	1762-1767 bp	0.5	59.8
tRNA-Val-tRNA-Val_p2	GAAATTGTAACCACGCACCG	CGAGCTAGGACACGAGTAGA	50.0	55.0	58.0	58.1	1641-1646 bp	0.5	59.4
tRNA-Val-tRNA-Val_p3	GCCCATAGGTTCGATTCCTG	TGCAGAGAAGGGTAGTGACA	55.0	50.0	58.1	58.0	1743-1748 bp	0.5	59.2
tRNA-Val-tRNA-Val_p4	GAAATTGTAACCACGCACCG	GCTTTCAGACGAGCTAGGAC	50.0	55.0	58.0	58.1	1650-1655 bp	0.5	59.2
tRNA-Val-tRNA-Val_p5	GAAATTGTAACCACGCACCG	TTGAACCAATGACTCCTGCC	50.0	50.0	58.0	58.1	1478-1483 bp	0.5	59.2