Hotspot

tRNA-Phe-tRNA-Val

Genome · 8837-11627 · 2791 bp

Back to Onobrychis

Pi 0.285

MarkerSeek score 78.60

Primer available yes

Label tRNA-Phe-tRNA-Val

Feature Metadata

label_name: tRNA-Phe-tRNA-Val
feature_type: igs
region: Genome
length_bp: 2791
variable_sites: 1204
pi: 0.285
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 78.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-Phe-tRNA-Val_p1	GGGATAGCTCAGCAGGTAGA	AGAAGGTCTACGGTTCGAGT	55.0	50.0	58.0	57.8	2890-2894 bp	0.5	58.6
tRNA-Phe-tRNA-Val_p2	AGAATGGTCGGGATAGCTCA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.9	57.8	2899-2903 bp	0.5	57.9
tRNA-Phe-tRNA-Val_p3	AGGACTGAAAATCCTCGTGC	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.9	57.8	2866-2870 bp	0.5	57.8
tRNA-Phe-tRNA-Val_p4	CAGGTAGAGCAGAGGACTGA	AGAAGGTCTACGGTTCGAGT	55.0	50.0	57.9	57.8	2878-2882 bp	0.5	57.5
tRNA-Phe-tRNA-Val_p5	GACTGAAAATCCTCGTGCCA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.2	57.8	2864-2868 bp	0.5	57.1