Hotspot

tRNA-Ser-tRNA-Thr

Genome · 5715-6816 · 1102 bp

Back to Onobrychis

Pi 0.3

MarkerSeek score 82.90

Primer available yes

Label tRNA-Ser-tRNA-Thr

Feature Metadata

label_name: tRNA-Ser-tRNA-Thr
feature_type: igs
region: Genome
length_bp: 1102
variable_sites: 502
pi: 0.3
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 82.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-Ser-tRNA-Thr_p1	TTGAATTGATTGTGTGCGCC	CTCAGAGGTTAGAGCATCGC	45.0	55.0	57.9	57.9	1332 bp	0.5	58.6
tRNA-Ser-tRNA-Thr_p2	TTGAATTGATTGTGTGCGCC	GATGGTCATCGGTTCGACTC	45.0	55.0	57.9	58.2	1301 bp	0.5	58.3
tRNA-Ser-tRNA-Thr_p3	ATGAGGCTGCATTAATCGGG	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.1	57.9	1809 bp	0.5	58.3
tRNA-Ser-tRNA-Thr_p4	CACTCGGTTTCTGGGCATAA	CTCAGAGGTTAGAGCATCGC	50.0	55.0	57.9	57.9	1285 bp	0.5	58.2
tRNA-Ser-tRNA-Thr_p5	ATGAGGCTGCATTAATCGGG	GATGGTCATCGGTTCGACTC	50.0	55.0	58.1	58.2	1778 bp	0.5	58.1