Hotspot

23S ribosomal RNA

Genome · 42776-45584 · 2809 bp

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Pi 0.26
MarkerSeek score 66.10
Primer available yes
Label 23S ribosomal RNA

Feature Metadata

label_name
23S ribosomal RNA
feature_type
rRNA
region
Genome
length_bp
2809
variable_sites
1101
pi
0.26
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.76
markerseek_score
66.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
23S ribosomal RNA_p1 GCCGTGACCTTCTCTTGAAT AGTAGCACCTTAGGATGGCA 50.0 50.0 57.9 58.1 2962 bp 0.5 58.2
23S ribosomal RNA_p2 ACTTCTTTTGCTCCATCCCC AGTAGCACCTTAGGATGGCA 50.0 50.0 57.8 58.1 2935 bp 0.5 57.3
23S ribosomal RNA_p3 GCCGTGACCTTCTCTTGAAT CCTTCAAGTTAAGGGCGAGG 50.0 55.0 57.9 58.3 2939 bp 0.5 57.1
23S ribosomal RNA_p4 ATTGCTGTCTCGGCTGTTAC AGTAGCACCTTAGGATGGCA 50.0 50.0 58.3 58.1 2898 bp 0.5 56.9
23S ribosomal RNA_p5 CATTGCTGTCTCGGCTGTTA AGTAGCACCTTAGGATGGCA 50.0 50.0 58.3 58.1 2899 bp 0.5 56.8