Hotspot

16S ribosomal RNA

Genome · 47957-49448 · 1492 bp

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Pi 0.326
MarkerSeek score 73.20
Primer available yes
Label 16S ribosomal RNA

Feature Metadata

label_name
16S ribosomal RNA
feature_type
rRNA
region
Genome
length_bp
1492
variable_sites
734
pi
0.326
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.90
markerseek_score
73.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
16S ribosomal RNA_p1 GAATTCTCGAGAGGGGAGGA CCGGTCCGGTTTTATGACAT 55.0 50.0 57.9 58.0 2502-2640 bp 0.5 59.1
16S ribosomal RNA_p2 GAATTCTCGAGAGGGGAGGA ATATCGACTCGGGTTCGTCT 55.0 50.0 57.9 58.0 2604-2742 bp 0.5 59.1
16S ribosomal RNA_p3 AATTCTCGAGAGGGGAGGAG CCGGTCCGGTTTTATGACAT 55.0 50.0 57.9 58.0 2501-2639 bp 0.5 59.1
16S ribosomal RNA_p4 AATTCTCGAGAGGGGAGGAG ATATCGACTCGGGTTCGTCT 55.0 50.0 57.9 58.0 2603-2741 bp 0.5 59.1
16S ribosomal RNA_p5 CGCCTCATCTATCCTCCTGA CCGGTCCGGTTTTATGACAT 55.0 50.0 58.1 58.0 2403-2541 bp 0.5 59.1