Hotspot

rpl14

LSC · 79095-79463 · 369 bp

Back to Oncidium

Pi 0.0217

MarkerSeek score 52.10

Primer available yes

Label rpl14

Feature Metadata

label_name: rpl14
feature_type: gene
region: LSC
length_bp: 369
variable_sites: 8
pi: 0.0217
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14_p1	GGTCGGCTGATACGCTTTAA	GCTCTTCAGGCACTAGAACC	50.0	55.0	58.1	58.0	1234-1263 bp	1.0	80.8
rpl14_p2	GGTCGGCTGATACGCTTTAA	GACAAATAGAAGCAGGGCGA	50.0	50.0	58.1	58.0	1197-1226 bp	1.0	80.8
rpl14_p3	GGTCGGCTGATACGCTTTAA	AGACAAATAGAAGCAGGGCG	50.0	50.0	58.1	58.0	1198-1227 bp	1.0	80.8
rpl14_p4	GGTCGGCTGATACGCTTTAA	CCTGCTTGGATCACAGCTAG	50.0	55.0	58.1	58.0	1216-1245 bp	1.0	80.6
rpl14_p5	GGTCGGCTGATACGCTTTAA	TCCGTTGTGAAACCGCTTTA	50.0	45.0	58.1	58.0	1048 bp	0.5	59.1