Hotspot

petB

LSC · 73274-74631 · 1358 bp

Back to Oncidium

Pi 0.00221

MarkerSeek score 60.00

Primer available yes

Label petB

Feature Metadata

label_name: petB
feature_type: gene
region: LSC
length_bp: 1358
variable_sites: 3
pi: 0.00221
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB_p1	TCTGGTAGTTCGATCGTGAA	ACTCTTGTTCCTCCCCGATA	45.0	50.0	56.0	57.5	1463 bp	1.0	60.1
petB_p2	TTCTGGTAGTTCGATCGTGA	ACTCTTGTTCCTCCCCGATA	45.0	50.0	56.0	57.5	1464 bp	1.0	60.1
petB_p3	CATTCTGGTAGTTCGATCGTGA	ACTCTTGTTCCTCCCCGATA	45.5	50.0	58.0	57.5	1466 bp	1.0	59.6
petB_p4	TCATTCTGGTAGTTCGATCGTG	ACTCTTGTTCCTCCCCGATA	45.5	50.0	58.0	57.5	1467 bp	1.0	59.6
petB_p5	TCTGGTAGTTCGATCGTGAAA	ACTCTTGTTCCTCCCCGATA	42.9	50.0	56.8	57.5	1463 bp	1.0	58.2