Hotspot

rpl14-rpl16

LSC · 79464-79583 · 120 bp

Back to Oncidium

Pi 0.0917

MarkerSeek score 71.70

Primer available yes

Label rpl14-rpl16

Feature Metadata

label_name: rpl14-rpl16
feature_type: igs
region: LSC
length_bp: 120
variable_sites: 11
pi: 0.0917
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14-rpl16_p1	TGGGCACTGCTTCTTTGATT	GACAAATAGAAGCAGGGCGA	45.0	50.0	58.0	58.0	528-558 bp	1.0	82.8
rpl14-rpl16_p2	TTGGGCACTGCTTCTTTGAT	GACAAATAGAAGCAGGGCGA	45.0	50.0	58.0	58.0	529-559 bp	1.0	82.7
rpl14-rpl16_p3	TGGGCACTGCTTCTTTGATT	AGACAAATAGAAGCAGGGCG	45.0	50.0	58.0	58.0	529-559 bp	1.0	82.7
rpl14-rpl16_p4	TGGGCACTGCTTCTTTGATT	GCTCTTCAGGCACTAGAACC	45.0	55.0	58.0	58.0	565-595 bp	1.0	82.6
rpl14-rpl16_p5	TTGGGCACTGCTTCTTTGAT	GCTCTTCAGGCACTAGAACC	45.0	55.0	58.0	58.0	566-596 bp	1.0	82.6