Hotspot

rpl20-rps12

LSC · 66929-67564 · 636 bp

Back to Oncidium

Pi 0.0206

MarkerSeek score 60.00

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 636
variable_sites: 13
pi: 0.0206
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	TCCGAGCTGTATATCCTCGT	CAGCGTCGAGGAACATGTAC	50.0	55.0	57.4	58.7	700-831 bp	1.0	71.5
rpl20-rps12_p2	TCCGAGCTGTATATCCTCGT	AGCGTCGAGGAACATGTACT	50.0	50.0	57.4	58.8	699-830 bp	1.0	70.8
rpl20-rps12_p3	TCCGAGCTGTATATCCTCGT	TCAGCGTCGAGGAACATGTA	50.0	50.0	57.4	58.8	701-832 bp	1.0	70.7
rpl20-rps12_p4	TCCGAGCTGTATATCCTCGT	GCGTCGAGGAACATGTACTA	50.0	50.0	57.4	57.2	698-829 bp	1.0	70.7
rpl20-rps12_p5	CGACGTCTCCGAGCTGTATA	CAGCGTCGAGGAACATGTAC	55.0	55.0	58.8	58.7	707-838 bp	1.0	69.7