Hotspot

accD-psaI

LSC · 57217-57663 · 447 bp

Back to Oncidium

Pi 0.0771

MarkerSeek score 73.90

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 447
variable_sites: 34
pi: 0.0771
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 73.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AGACAGTACCTGACGGTTCA	AAAGAAGCCATTGCGATTGC	50.0	45.0	58.0	58.0	667-1057 bp	1.0	84.0
accD-psaI_p2	AATGCCTACATTGCGTTTGC	AAAGAAGCCATTGCGATTGC	45.0	45.0	58.0	58.0	719-1109 bp	1.0	83.8
accD-psaI_p3	CCTACAACTGGCGGAGTTAC	AAAGAAGCCATTGCGATTGC	55.0	45.0	58.0	58.0	782-1172 bp	1.0	83.6
accD-psaI_p4	AATGCCTACATTGCGTTTGC	TCAACTAGCAGCATTCCGAC	45.0	50.0	58.0	58.0	1057-1448 bp	1.0	82.7
accD-psaI_p5	CCTACAACTGGCGGAGTTAC	TCAACTAGCAGCATTCCGAC	55.0	50.0	58.0	58.0	1120-1511 bp	1.0	82.5