Hotspot

ndhJ-ndhC

LSC · 48564-48664 · 101 bp

Back to Oncidium

Pi 0.0891

MarkerSeek score 80.60

Primer available yes

Label ndhJ-ndhC

Feature Metadata

label_name: ndhJ-ndhC
feature_type: igs
region: LSC
length_bp: 101
variable_sites: 9
pi: 0.0891
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhJ-ndhC_p1	TATCCCTCGGCAATCAAAGC	AAAAGGAGCGTTGGAATGGT	50.0	45.0	58.0	58.0	202 bp	1.0	83.3
ndhJ-ndhC_p2	AAAGTCTCTATCCCTCGGCA	AAAAGGAGCGTTGGAATGGT	50.0	45.0	57.8	58.0	210 bp	1.0	82.1
ndhJ-ndhC_p3	AAGTCTCTATCCCTCGGCAA	AAAAGGAGCGTTGGAATGGT	50.0	45.0	57.8	58.0	209 bp	1.0	82.1
ndhJ-ndhC_p4	TATCCCTCGGCAATCAAAGC	GGTTCAGTTTATGCATGGCG	50.0	50.0	58.0	57.8	222 bp	1.0	81.4
ndhJ-ndhC_p5	TATCCCTCGGCAATCAAAGC	CGAAAAGGAGCGTTGGAATG	50.0	50.0	58.0	57.8	204 bp	1.0	81.3