Hotspot

trnT-GGU-psbD

LSC · 31725-32662 · 938 bp

Back to Oncidium

Pi 0.00961

MarkerSeek score 61.40

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 938
variable_sites: 9
pi: 0.00961
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	GGACCAGCCTACAAAAACGA	55.0	50.0	58.1	58.1	1097-1098 bp	1.0	79.3
trnT-GGU-psbD_p2	TCAGTGGTAGAGTAACGCCA	GGACCAGCCTACAAAAACGA	50.0	50.0	58.1	58.1	1100-1101 bp	1.0	79.1
trnT-GGU-psbD_p3	GTGGTAGAGTAACGCCATGG	ACAAAAACGAAACGGTCCCT	55.0	45.0	58.1	58.2	1087-1088 bp	1.0	78.3
trnT-GGU-psbD_p4	GTGGTAGAGTAACGCCATGG	CCCTCCGTAACCAATCATCC	55.0	55.0	58.1	57.7	1071-1072 bp	1.0	78.2
trnT-GGU-psbD_p5	TCAGTGGTAGAGTAACGCCA	ACAAAAACGAAACGGTCCCT	50.0	45.0	58.1	58.2	1090-1091 bp	1.0	78.1