Hotspot

ndhD

SSC · 115393-116913 · 1521 bp

Back to Omphalogramma

Pi 0.00824

MarkerSeek score 51.90

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1521
variable_sites: 34
pi: 0.00824
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	GCCAATTCTTCGATTGTGGC	AAATTGCTTCTGCCCCAAGA	50.0	45.0	58.1	58.0	2134-2142 bp	1.0	81.6
ndhD_p2	GCCAATTCTTCGATTGTGGC	AATTGCTTCTGCCCCAAGAA	50.0	45.0	58.1	58.0	2133-2141 bp	1.0	81.6
ndhD_p3	TGGCTTCGATTGGCTTTCTT	AAATTGCTTCTGCCCCAAGA	45.0	45.0	58.1	58.0	2118-2126 bp	1.0	81.5
ndhD_p4	TGGCTTCGATTGGCTTTCTT	AATTGCTTCTGCCCCAAGAA	45.0	45.0	58.1	58.0	2117-2125 bp	1.0	81.5
ndhD_p5	GCCAATTCTTCGATTGTGGC	AGCATGGGTCTCGCTTATTG	50.0	50.0	58.1	58.0	2021-2029 bp	1.0	81.5