Hotspot

trnL-UAG-ccsA

SSC · 114056-114161 · 106 bp

Back to Omphalogramma

Pi 0.0289

MarkerSeek score 64.30

Primer available yes

Label trnL-UAG-ccsA

Feature Metadata

label_name: trnL-UAG-ccsA
feature_type: igs
region: SSC
length_bp: 106
variable_sites: 8
pi: 0.0289
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-ccsA_p1	TAGACACGCTGCTCTTAGGA	AGGAAATATGAGTTAATATATGCTCT	50.0	26.9	57.9	54.0	209-211 bp	1.0	43.4
trnL-UAG-ccsA_p2	AAATTGGTAGACACGCTGCT	AGGAAATATGAGTTAATATATGCTCT	45.0	26.9	57.8	54.0	216-218 bp	1.0	43.3
trnL-UAG-ccsA_p3	AACAAGCCGCTATGGTGAAA	AGGAAATATGAGTTAATATATGCTCT	45.0	26.9	58.1	54.0	233-235 bp	1.0	43.1
trnL-UAG-ccsA_p4	ACAAGCCGCTATGGTGAAAT	AGGAAATATGAGTTAATATATGCTCT	45.0	26.9	57.9	54.0	232-234 bp	1.0	43.1
trnL-UAG-ccsA_p5	TTTGAACAAGCCGCTATGGT	AGGAAATATGAGTTAATATATGCTCT	45.0	26.9	58.1	54.0	237-239 bp	1.0	43.0