Hotspot

ndhF-rpl32

SSC · 112513-113384 · 872 bp

Back to Omphalogramma

Pi 0.0213

MarkerSeek score 72.90

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 872
variable_sites: 48
pi: 0.0213
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	AAGAAGAAGTCCCACTCCGA	AAAGCCGCCCAATATCCTTT	50.0	45.0	58.0	57.8	1036-1045 bp	1.0	84.3
ndhF-rpl32_p2	AAGAAGAAGTCCCACTCCGA	AAGCCGCCCAATATCCTTTC	50.0	50.0	58.0	58.3	1035-1044 bp	1.0	83.1
ndhF-rpl32_p3	AAGAAGAAGTCCCACTCCGA	GCCGCCCAATATCCTTTCTT	50.0	50.0	58.0	58.3	1033-1042 bp	1.0	83.1
ndhF-rpl32_p4	AGAAGAAGTCCCACTCCGAT	AAAGCCGCCCAATATCCTTT	50.0	45.0	57.8	57.8	1035-1044 bp	1.0	82.5
ndhF-rpl32_p5	AGAAGAAGTCCCACTCCGAT	GCCGCCCAATATCCTTTCTT	50.0	50.0	57.8	58.3	1032-1041 bp	1.0	81.3